Department of Cell Signal Systems

The research program covers the areas:

Studies on peculiarities of PI3K/S6K dependent signal pathway in mammalian cells under normal and pathological conditions (malignant transformation of cells).

Identification and characterisation of tumour-associated antigens of human using the SEREX (Serologic identification of Recombinant Expressed antigens) analysis.

Investigation of molecular mechanism of pathogenesis of dilated cardiomyopathy (DCM) as autoimmune cardio-specific pathology with mitochondrial dysfunction.

Studies on the properties of common cytoplasmic and mitochondrial molecular chaperons at malignant and non-malignant neoplasms in humans, as well as autoimmune and autoimmune-dependent pathologies.

Ongoing research projects:

• The peculiarities of two isoforms of ribosomal protein kinase S6 (S6K1 and S6K2) in both normal tissue and malignant tumours (adenocarcinoma of mammary gland of human) are under investigation. The analysis of expression level and the features of subcellular localisation is being performed.
• Identification of novel proteins-partners of the components of PI3K/S6K dependent signal pathway (S6K, PTEN, TSC1/TSC2) using the method of the double - hybrid system of leaven and the analysis of intermolecular interactions.
• Cloning of KoA synthase gene. Analysis of the interaction between KoA synthase and S6K1.
• Expression and subcellular localisation of molecular chaperons of Hsp60, Hsp70, Hsp90 and Hsp27 at oncological and autoimmune diseases of humans are under examination.
• Monoclonal antibodies for the components of signal pathways and molecular chaperones have been obtained.

Selected publications:

1. I.Gout , T.Minami, K. Hara, Y.Tsujishita, V.Filonenko, et al. Molecular cloning and characterization of a novel p70 S6 kinase, p70 kinase b, containing a prolin-rich region // J.Biol.Chem - 1998, -V.273, №46. - Р.30061-64.
2. Zhyvoloup A, Nemazanyy I, Babich A, Panasyuk et al Molecular cloning of CoA Synthase. The missing link in CoA biosynthesis // J. Biol. Chem. – 2002. – V.277, №25. – P.22107-10.
3. Zhyvoloup A., Nemazanyy I., Panasyuk G., Valovka et al Subcellular localization and regulation of coenzyme A synthase // J. Biol. Chem. – 2003. – V.278, № 50. – P.50316-21.
4. Savinska L. O., Lyzogubov V. V., Usenko V. S., Ovcharenko G. V. et al. Immunohistochemical analysis of S6K1 and S6K2 expression in human breast tumors // Experimental Oncology.–2004.–V.26, №1. – P.24-30.
5. Ponomarenko N.A., Durova O.M., Vorobiev I.I.et.al. Catalytic antibodies in clinical and experimental pathology: Human and mouse models // J.Immunol.Methods. – 2002. – v.269. – P.197-211.
6. Vigontina O.G., Efimenko O.A., Yakovenko L.F. et.al. Chaperon Hsp60 as autoantigen in development of dyshormonal breast diseases // Experimental Oncology. – 2002. – Vol.24, № 2. – Р.112-115.