Head

Professor Anna V. El’skaya, Dr.Sc., Member of National Academy of Sciences of Ukraine

Phone: (380-44) 526- 07-49;
Fax: (380-44) 526-07-59;
E-mail: elskaya@imbg.org.ua

Laboratory of Biomolecular Electronics

Head Professor Olexey P. Soldatkin, Dr.Sc.

Laboratory of Protein Biosynthesis

Head Professor Boris S. Negrutskii, Dr.Sc.

The fundamental research of the Department encompasses:

The elaboration of novel analytical systems, bio- and chemosensors, on the basis of different electrochemical transducers and biological objects or synthetic biomimics

Ongoing research projects are focused on:

The elongation stage of protein biosynthesis on higher eukaryotic ribosomes is being studied

Investigation of the tissue- and cycle-specific genes expression in the actively proliferating eukaryotic cells or the cells under the influence of unfavourable conditions (low dose radiation, chemical pollution etc.)

Much attention is given to test-systems for the herbicides and pesticides determination, to the development of new enzyme analytical sets, enzyme – and cell biosensors for the monitoring of formaldehude, heavy metals ions and other toxic substances.

Studies on biomimics, which have biological activity but at the same time, being more stable, they are suitable for analytical devices in the conditions when the biological elements of sensors get inactivated.

Selected publications:

1. Budkevich TV, El'skaya AV, Nierhaus KH. Features of 80S mammalian ribosome and its subunits. Nucleic Acids Res. 2008 ; 36 (14) : 4736-44.
2. Panasyuk G., Nemazanyy I., Filonenko V., Negrutskii B., El'skaya A. A2 isoform of mammalian translation factor eEF1A displays increased tyrosine phosphorylation and ability to interact with different signalling molecules Int J Biochem Cell Biol. 2008 ; 40 (1): 63-71.
3. Kanibolotsky DS, Novosyl'na OV, Abbott CM, Negrutskii BS, El'skaya AV. Multiple molecular dynamics simulation of the isoforms of human translation elongation factor 1A reveals reversible fluctuations between "open" and "closed" conformations and suggests specific for eEF1A1 affinity for Ca2+-calmodulin. BMC Struct Biol. 2008; 8:4.
4. S.V.Dzyadevych, V.N.Arkhypova, A.P.Soldatkin, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Conductometric enzyme biosensors. Handbook of Biosensors and Biochips / Eds R..S.Marks, C.R.Lowe, D.C.Culen, H.H.Weetall, I.Karube.- Wiley-Interscience.- 2007.- P. 379-394.
5. S.V.Dzyadevych, A.P.Soldatkin, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Enzyme biosensors based on ion-selective field-effect transistors. Analytica Chimica Acta.- 2006, 568.- P.248-258.
6. A.P.Soldatkin, S.V.Dzyadevych, A.V.El’skaya, C.Martelet, N.Jaffrezic-Renault. Pathways for improving potentiometric and conductometric enzymatic biosensors. In: Encyclopedia of Sensors (Eds. C.A.Grimes, E.C.Dickey, M.V.Pishko).- California, USA: American Scientific Publishers.- 2006.- V.7.- P. 331-348.
7. S.V.Dzyadevych, A.P.Soldatkin, V.N.Arkhypova, C.Martelet, N.Jaffrezic-Renault, A.V.El’skaya. Electrochemical enzyme biosensors. Kyiv: IMBG.- 2006.- 72 p.
8. A.P.Soldatkin, S.V.Dzyadevych, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault, Pathways for improving potentiometric and conductometric enzymatic biosensors, Encyclopedia of Sensors, American Scientific Publishers, submitted, 2005
9. A.P.Soldatkin, V.N.Arkhypova, S.V.Dzyadevych, A.V.El'skaya, J.-M.Gravoueille, N.Jaffrezic-Renault, C.Martelet, Analysis of the potato glycoalkaloids by using of enzyme biosensor based on pH-ISFETs, Talanta. - 2005. -66 -P.28-33
10. S.V.Dzyadevych, A.P.Soldatkin, V.N.Arkhypova, A.V.El'skaya, J.-M. Chovelon, C.Georgiou, C.Martelet, N.Jaffrezic-Renault, Early-warning electrochemical biosensor system for the environmental monitoring based on enzyme inhibition effect, Sensors and Actuators B.- 2004
11. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, Y.I.Korpan, A.V.El'skaya, J.-M.Gravoueille, C.Martelet, N.Jaffrezic-Renault, Application of enzyme field effect transistors for fast detection of total glycoalkaloids content in potatoes, Sensors and Actuators B.- 2004.- 103.- P. 416-422
12. O.A.Biloivan, S.V.Dzyadevych, O.A.Boubryak, A.P.Soldatkin, A.V.El'skaya, Development of enzyme biosensor based on ISFETs for quantitative analysis of serine proteinases, Electroanalysis.- 2004.- 16.- P. 1883-1889
13. Lukash, T.O. Turkivska H.V., Negrutskii B.S., El'skaya A.V., Chaperone-like activity of mammalian elongation factor eEF1A: Renaturation of aminoacyl-tRNA synthetases", International Journal of Biochemistry & Cell Biology -2004, V.36 P. 1341-1347
14. Korpan Y.I., Nazarenko E.A., Skryshevskaya I.V., Martelet C., Jaffrezic-Renault N., El'skaya A.V., Potato Glycoalkaloids: True Safety or False Sense of Security?, Trends in Biotechnology.- 2004. -22, N3. -P.147-151
15. S.V.Dzyadevych, V.N.Arkhypova, A.P.Soldatkin, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault, Enzyme biosensor for tomatine detection in tomatoes, Analytical Letters.- 2004. - 37, No 8.- P. 1611-1624
16. S.V.Dzyadevych, V.N.Arkhypova, C.Martelet, N.Jaffrezic-Renault, J.-M.Chovelon, A.V.El'skaya, A.P.Soldatkin, Potentiometric biosensors based on ISFETs and immobilized cholinesterases, Electroanalysis. - 2004. - 16. - P. 1873-1882.
17. T.V. Budkevich, A.A. Timchenko, E.I. Tiktopulo, B.S. Negrutskii, V.F. Shalak, Z.M. Petrushenko, V.L. Aksenov, R. Willumeit, J. Kohlbrecher, I.N.Serdyuk, A.V.El'skaya, Extended conformation of mammalian translation elongation factor 1A in solution, Biochemistry. -2003. - 24. -N51. -P.15342-15349.
18. T.A.Sergeyeva, S.A.Piletsky, E.V.Piletska, O.O.Brovko, L.V.Karabanova, L.M.Sergeeva, A.V.El'skaya, A.P.F.Turner, In situ formation of porous molecularly imprinted polymer membranes, Macromolecules.-2003.- 36.- N19. -P. 7352-7357.
19. V.N.Arkhypova, S.V.Dzyadevych, A.P.Soldatkin, A.V.El'skaya, C.Martelet, N.Jaffrezic-Renault, Development and optimisation of biosensors based on pH-sensitive field effect transistor and cholinesterase for sensitive detection of solanaceous glycoalkaloids, Biosensors & Bioelectronics.- 2003.- 18.- P. 1047-1053.
20. S.V.Dzyadevych, A.P.Soldatkin, Y.I.Korpan, V.N.Arkhypova, A.V.El'skaya, J.-M.Chovelon, C.Martelet, N.Jaffrezic-Renault, Biosensors based on enzyme field effect transistors for determination of some substrates and inhibitors, Analytical and Bioanalytical Chemistry.- 2003.- 377.- P. 496-506
21. Petrushenko Z.M., Budkevich T.V., Shalak V.F., Negrutskii B.S., El'skaya A.V. Novel complexes of mammalian translation elongation factor eEF1A*GDP with uncharged tRNA and aminoacyl-tRNA synthetase. Implications for tRNA channeling // Eur. J. Biochem. - 2002. -V.269. - P. 4811-4818.
22. Budkevich, T.V., Timchenko, A. A., Tiktopulo, E.I., Negrutskii, B.S., Shalak, V.F., Petrushenko, Z.M., Aksenov, V.L., Willumeit, R., Kohlbrecher, J., Serdyuk, I.N., El'skaya A.V. Extended conformation of mammalian translation elongation factor 1A in solution // Biochemistry. - 2002. - V. 41. - P. 15342-15349.